Evaluation of contaminant kinetics in tetra-pack conditioned juice: evolution of the physico-chemical and microbiological parameters during the storage period

F. Lecheb, S. Bougherara, K. Delleci, L. Djoubani, M. Lazazi, B. Rahal


Abstract: Packing is the new operations in the manufacture of food products, they will help maintain the hygiene, tactile and nutritional quality of the food and meet the logistical constraints. A study was conducted to determine the effects of packaging materials, storage temperature, and time on physiochemical and bacteriological properties of conditioned juice. pH, acidity, Brix, mineral content (heavy metals before and after contact, in packaging and in juice), migration test, microbiological analysis, and antioxidant activity (FRAP) were evaluated in freshly prepared juice, and after 15, 30, 45, 60, 75, and 90 days of storage at room temperature. A migration test was conducted using three percent acetic acid as a simulator followed by an Atomic Flamming Adsorption Spectroscopy analysis of the tested packaging and analysis by TF- IR of the product. The results showed that pH, acidity, Brix, decreased with storage times. According to the results obtained no variability was observed for the microbiological parameters during the storage period. As for the physicochemical parameters, minor variations have been observed. The loss of total phenols and vitamin C content was higher in juice stored at 90 days than 15 days. The results of mineral analyzes show a slight variation of 1 to 2 mg / l compared to the reference sample (0-day storage sample), for K +, Na +, Cl- and PO4-3, with the exception of calcium ion, for which there was a sharp increase after 45 days of storage, which then declined. The findings showed the lack of migration of the packaging to the food, thus maintaining the initial composition.

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ALINORM 83/12A. Programme mixte FAO/OMS sur les norms alimentaires Commission du codex alimentarius. Quinzième session Rome, 4-15 juillet 1983. Rapport de la seizième session du comité du codex sur les additifes alimentaires.

ALINORM 05/28/39. 2005. Programme mixte FAO/OMS sur les normes alimentaires. Commission de codex alimentarius. Vingt-huitième session Rome (Italie), 4 - 9 juillet 2005. Rapport de la quatrième session du groupe intergouvernementale spécial du codex sur les jus de fruits et de légumes Fortaleza, Ceará (Brésil).

Arfa, D. Suivi des caractéristiques microbiologique et physico-chimique des jus des dattes conservés par irradiation gamma. Thèse de technicien supérieur en Industrie agroalimentaire (2008).

Askar, A. Flavor changes during processing and storage of fruit juices-II. Interaction with packaging materials. Fruit Processing, 11 (1999) 432–439.

Ashworth, D.J.; Alloway, B J. Environmental Pollut. 127 (2004) 137-144.

ATSDR. Toxicological profile for Zinc. US Department of health & human services. Public health service. Agency for toxic substances and disease registry (2005).

Ayhan, Z.; Yeom, H .; W., Zhang, Q H.; Min, D.B. Flavor, color, and vitamin C retention of pulsed electric field processed orange juice in different packaging materials. J. Agric. Food Chem., 49 (2001), 669–674.

Beliles, R P. The metals. In: Patty’s Industrial Hygiene and Toxicology, Fourth edition, Volume 2, Part C (1994).

Berlinet, C. Etude de l'influence de l'emballage et de la matrice sur la qualite du jus d'orange. Sciences du Vivant [q-bio]. ENSIA (AgroParisTech), Français (2006).

Bourokaa A. Etude biochimique de l'adulteration du jus de fruits. Université de Carthage - Microthèse. Institut National des Sciences Appliquées et de Technologie (2012).

Codex Stan . Norme codex pour les oranges (2004).

Commission Européenne. Directive 98/83/CE du Conseil du 3 Novembre 2004 relative à la qualité des eaux destinées à la consommation humaine (2004).

Echeverria, E.; Valich, J. Enzymes of sugar and acid metabolism in stored Valencia oranges. Journal of the American Society for Horticultural Science.114 (1989) 445-449.

EDQM (Direction européenne de la qualité du medicament et soins de santé). (2014). Métaux et alliage constitutifs des matériaux et objets pour contact alimentaire.

Esteve, M. J.; Frıgola, A.; Rodrigo, C.; Rodrigo, D. Effect of storage period under variable conditions on the chemical and physical composition and colour of Spanish refrigerated oranage juices. Food and Chemical Toxicology, 43 (2005) 1413–1422.

EU-RAR (European Risk Assessment Report).. Copper Voluntary Risk Assessment Report.European Copper Institute (2008).

EVM. Expert Group on Vitamins and Minerals. Review of Chromium (2002).

FAO/OMS. Codex alimentarius. Programme mixte FAO/OMS sur les normes alimentaires. Commission de codex alimentarius. Rapport de la Trente-sixième session du comité du codex sur les additifs alimentaires et les contaminants. 15p (2003).

Fan, Y-Y.; Zheng, J-L.; Ren, J-H.; Luo, J.; Cui, X-Y.; Ma, LQ. Effects of storage temperature and duration on release of antimony and bisphenol A from polyethylene terephthalate drinking water bottles of China. Environnement Pollutution. 192(2014)113–20.

Fayoux, S C.; Seuvre, A M.; Voilley, A J.;. Aroma transfer in and through plastic packagings: Orange juice and d-limonene. A review. Part I: Orange juice aroma sorption. Packaging Technology and Science, 10 (1997) 69–82.

Feigenbaum, A. ; Barthélémy E. Evaluation sanitaire des matériaux en poly (éthylène téréphtalate) recyclés utilisés en tant que matériaux au contact des denrées alimentaires et de l’eau de boisson (2006).

Friberg, L.; Kjellström, T.; Nordberg, G.F. Cadmium. In: Friberg, L., Nordberg, G.F., Vouk, V.B. Handbook on the toxicology of metals. Second Edition. Elsevier, Amsterdam, New York, Oxford (1986).

Fathabad, AE.; Shariatifar, N.; Moazzen, M. Nazmara, S.; Fakhri, Y.; Alimohammadi, M. . Determination of heavy metal content of processed fruit products from Tehran’s market using ICP-OES: a risk assessment study. Food Chem Toxicol.;115 (2018) 436–46.

Grażyna, K.; Urszula, P.; Radosław, K.; Artur, M. Determination of the content of selected trace elements in Polish commercial fruit juices and health risk assessment. Open Chemistry ; 18 (2020) 443–452

Huelin, F E.; Coggiola, I M.; Sidhu, G C.,; Kennett, H. The anaerobic decomposition of ascorbic acid in the pH range of foods and in more acid solution. Journal of the Science of Food and Agriculture. 22(1971) 540-542.

Kabasakalis, V. ; Siopidou, D. ; Moshatou, E. Ascorbic acid content of commercial fruit juices and its rate of loss upon storage. Food Chemistry, 70(2000) 325–328.

Konkol, L. Contaminants levels in recycled PET plastic, Swinburne University of Technology, Victoria (Australia) (2004). Thesis.

Khoudali, S. ; Benmessaoud, D. ; Essaqui, A. ; Zertoubi, M. ; Azzi, M. ; Benaissa, M. Equipe de Chimie Agroalimentaire et Chimie des substances Naturelles Végétales et Marines, Faculté des Sciences Ain Chock, Université Hassan II-Casablanca – Morocco (2013).

Leland, J.V. Flavor Interactions: The Great Whole. Food Technology 51 (1997)) 75-80.

Miller, D M.; Miller, W P. Land application of waste. Summer M.E (ed) Handbook of soil (2000).

Nagy, S.; Shaw, P E. Factors Affecting The Flavour of citrus Fruit. In: Morton, I. D.; MacLeod, A.J. (Eds.). Food Flavours. Part C: The Flavour of Fruits. Elseiver. (1990) 93-124.

Nielsen, T. ; Jagerstad, M. Flavour scalping by food packaging. Trends food science and technology (1994), vol 5.

Nordic Council of Ministers. Risk evaluation of essential trace elements – essential versus toxic levels of intake. Report of a Nordic project group. Ed.: Oskarsson, A. Nordic Council of Ministers, (1995) Copenhagen, Denmark.

OFSP (Office fédéral de la santé publique). Animoine dans les denrées alimentaires et repas de commodité conditionnés en barquettes de PET (2007).

Okoronkow, N E.; Igwe, J C.; Nwuchekwa, E C. African Journal of Biotechnology Vol. 4 (13) (Dec. 2005) 1521-1524.

Panisset, J. C. ; Dewailly, E. ; Doucet-Leduc, H. Contamination alimentaire. In: Environnement et santé publique-Fondements et pratiques (2003) 369-395.

Park, G. L., Byers J. L., Pritz C. M., Nelson D. B.; Navarro, J. L.; Smolensky, D. C.; Vandercook, C. Characteristics of California Navel Orange Juice and Pulpwash. Journal of Food Science. 48 (1983) 627-632

Polydera, A. C.; Stoforos, N. G. ; Taoukis, P. S. Comparative shelf life study and vitamin C loss kinetics in pasteurised and high pressure processed reconstituted orange juice, Journal of Food Engineering, 60 (2003) 21-29.

Règlement (CE) N°1935/2004 du parlement européen et du conseil du 27 octobre 2004, concerned les métaux et objet destines à entrer en contacte avec des denrées alimentaires et abrogeant les directives80/590/CEE.

Rodushkin, I.; Magnusson, A. Aluminium migration to orange juice in laminated paperboard packages. Journal of food composition and analysis. 18(2005) 365–74.

Rouseff, R.; Nagy, S. A Multivariate Pattern Recognition Approach for Identifying Quality Factors in Florida Orange Juice. In: Martens. Flavour Science and Technology. (1987) 481- 488.

SCF on 22 november 2000. Guidelines of the Scientific Committee on Food for the presentation of an application for the safety assessment of a substance to be used in food contact materials prior to its authorisation. SCF/CS/PLEN/GEN/90 Final, 8 December 2000.

Siegmund, B.; Derler, K.; Pfannhauser, W. Chemical and sensory effects of glass and laminated carton packages on fruit juice products—Still a controversial topic. lebensm wiss u technology journal, 37(2004) 481–488.

Sizer, C. E.; Waugh, P. L.; Edstam, S.; Ackermann, P. Maintaining flavor and nutrient quality ofaseptic orange juice— storage temperature is one of the primary factors affecting stability. Food Technology. 42 (1988) 152–159.

Tasnim, F.; Hossain, M.A.; Nusrath, S.; Hossain, K.M.; Lopa, D.; Formuzul Haque, K.M. Quality Assessment of Industrially Processed Fruit Juices Available in Dhaka City. Malaysian Journal of Nutrition, 16(2010) 431-438.

Vousta, D.; Grimarins, A.; Sammara, C. Environ. Pollut.; 94(3) (1996) 325-335.

Zerdin, K.; Rooney, M. L.; Vermue, J. The vitamin C content of orange juice packed in an oxygen scavenger. Material. Food Chemistry, 82 (2003) 387–395.


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